PROJECT MEMBERS

Dr. Simon Trowitzsch
Group Leader
phone +49-(0)69-798-29274

Andreas Blees
PhD Student
phone: +49-(0)-69-798-29502

Nicole Koller
PhD Student
phone: +49-(0)-69-798-29505

Alexander Domnick
PhD Student
phone: +49-(0)-69-798-29502

Jamina Brunnberg
PhD Student
phone: +49-(0)-69-798-29505

Ines Katharina Müller
PhD Student
phone: +49-(0)-69 - 798 29502

Molecular Architecture of the Peptide-Loading Complex

The peptide-loading complex (PLC) is a key player in the adaptive immune system. This highly dynamic multiprotein membrane complex ensures that appropriate, high-affinity peptides are loaded onto Major Histocompatibility Complex (MHC I) class I molecules for cell-surface presentation to cytotoxic T-cells. We have recently developed fluorescence-based tools for tracking recombinant production and purification of multimeric membrane complexes in bacterial or eukaryotic expression hosts. In order to derive detailed mechanistic insights into how optimal peptide loading is accomplished, we combine molecular, cell and structural biology with immunological and biophysical techniques.

Publications:

Blees A, Januliene D, Hofmann T, Koller N, Schmidt C, Trowitzsch S, Moeller A, Tampé R.
Structure of the human MHC-I peptide-loading complex.
Nature. 2017 Nov 6. doi: 10.1038/nature24627.   PDF

Thomas C, Tampé R.
Structure of the TAPBPR-MHC I complex defines the mechanism of peptide loading and editing.
Science. 2017 Oct 12. pii: eaao6001. doi: 10.1126/science.aao6001.  PDF

Hulpke S, Tampé R. (2013)
The MHC I loading complex: a multitasking machinery in adaptive immunity.
Trends Biochem Sci. 2013 Aug;38(8):412-20.   PDF

Hulpke S, Baldauf C, Tampé R.
Molecular architecture of the MHC I peptide-loading complex – one tapasin molecule is essential and sufficient for antigen processing.
FASEB J. 2012 Dec;26(12):5071-80   PDF

Hulpke S, Tomioka M, Kremmer E, Ueda K, Abele R, Tampé R. (2012) Direct evidence that the N-terminal extensions of the TAP complex act as autonomous interaction scaffolds for the assembly of the MHC I peptide-loading complex.
Cell Mol Life Sci. 2012 May 27.  PDF