PROJECT MEMBERS

Dr. Simon Trowitzsch
Group Leader
phone +49-(0)69-798-29274

Andreas Blees
PhD Student
phone: +49-(0)-69-798-29502

Nicole Koller
PhD Student
phone: +49-(0)-69-798-29505

Alexander Domnick
PhD Student
phone: +49-(0)-69-798-29502

Jamina Brunnberg
PhD Student
phone: +49-(0)-69-798-29505

Ines Katharina Müller
PhD Student
phone: +49-(0)-69 - 798 29502

Molecular Architecture of the Peptide-Loading Complex

The peptide-loading complex (PLC) is a key player in the adaptive immune system. This highly dynamic multiprotein membrane complex ensures that appropriate, high-affinity peptides are loaded onto Major Histocompatibility Complex (MHC I) class I molecules for cell-surface presentation to cytotoxic T-cells. We have recently developed fluorescence-based tools for tracking recombinant production and purification of multimeric membrane complexes in bacterial or eukaryotic expression hosts. In order to derive detailed mechanistic insights into how optimal peptide loading is accomplished, we combine molecular, cell and structural biology with immunological and biophysical techniques.

Publications:

Seyffer F, Tampé R. (2014)
ABC transporters in adaptive immunity.
Biochim Biophys Acta. 2014 Jun 9. pii: S0304-4165(14)00220-7.   PDF

Hulpke S, Tampé R. (2013)
The MHC I loading complex: a multitasking machinery in adaptive immunity.
Trends Biochem Sci. 2013 Aug;38(8):412-20.   PDF

Hinz A, Jedamzick J, Herbring V, Fischbach H, Hartmann J, Parcej D, Koch J, Tampé R.
Assembly and function of the MHC I peptide-loading complex are conserved across higher vertebrates
J. Biol. Chem. 2014 Oct 15; 289: 33109-33117.   PDF

Parcej D, Guntrum R, Schmidt S, Hinz A, Tampé R. (2013)
Multicolour Fluorescence-Detection Size-Exclusion Chromatography for Structural Genomics of Membrane Multiprotein Complexe
PLoS One. 2013 Jun 25;8(6):e67112   PDF

Hulpke S, Baldauf C, Tampé R.
Molecular architecture of the MHC I peptide-loading complex – one tapasin molecule is essential and sufficient for antigen processing.
FASEB J. 2012 Dec;26(12):5071-80   PDF

Hulpke S, Tomioka M, Kremmer E, Ueda K, Abele R, Tampé R. (2012) Direct evidence that the N-terminal extensions of the TAP complex act as autonomous interaction scaffolds for the assembly of the MHC I peptide-loading complex.
Cell Mol Life Sci. 2012 May 27.   PDF

Abele R, Tampé R.
The TAP translocation machinery in adaptive immunity and viral escape mechanisms.
Essays Biochem. 2011 Sep 7;50(1):249-64   PDF