Project Members

PD Dr. Rupert Abele
Group Leader
phone +49-(0)69-798-29437

Christoph Bock
PhD Student
phone: +49-(0)-69-798-29474

Philipp Graab
PhD Student
phone: +49-(0)-69-798-29474

Lysosomal Peptide Translocation

Lyosomes are the trash bin of the cell, in which macromolecules are degraded to single building blocks. Different pathways exist for the delivery of substrates to lysosomes. We have identified the recently discovered ATP-binding-cassette (ABC) transporter ABCB9 as peptide transporter which translocates cytosolic peptides into the lumen of the lysosomes. In future, we will study the physiological function and the subcellular targeting mechanism by immunological and cell biological methods. The molecular transport mechanism and structure of this peptide transporter will be analyzed by biochemical and biophysical approaches including single molecule techniques.


Publications:

Tumulka F, Roos C, Löhr F, Bock C, Bernhard F, Dötsch V, Abele R.
Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.
J Biomol NMR. 2013 Oct;57(2):141-54.

Bangert I, Tumulka F, Abele R. (2011) The lysosomal polypeptide transporter TAPL: more than a housekeeping factor?
Biol Chem. 2011 Jan;392(1-2):61-6.   PDF

Demirel O, Jan I, Wolters D, Blanz J, Saftig P, Tampé R, Abele R.
The lysosomal polypeptide transporter TAPL is stabilized by the interaction with LAMP-1 and LAMP-2. J Cell Sci. 2012 May 28.    PDF

Demirel Ö, Bangert I, Tampé R, Abele R. (2010) Tuning the cellular trafficking of the lysosomal peptide transporter TAPL by its N-terminal domain.
Traffic. 2010 Mar;11(3):383-93.   PDF

Zhao C, Haase W, Tampé R, Abele R. (2008) Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL).
J Biol Chem 283, 17083-17091.     PDF

Demirel O, Waibler Z, Kalinke U, Grunebach F, Appel S, Brossart P, Hasilik A, Tampé R,  Abele R. (2007) Identification of a lysosomal peptide transport system induced during dendritic cell development.
J Biol Chem 282, 37836-37843.     PDF

Wolters JC, Abele R, Tampé R. (2005) Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9). J Biol Chem 280, 23631-23636.     PDF