Project Members

Dr. Robert Tampé
Professor & Director   
phone +49-(0)69-798-29476

Stefan Brüchert
PhD Student
phone: +49-(0)-69-798-29471

Philipp Höllthaler
PhD Student
phone: +49-(0)-69-798-29470

Mitochondrial ABC Proteins in Health and Diseases

Function of the Mitochondrial ABC Proteins

ABC transporters represent one of the largest families of membrane proteins that are found in all three phyla of life. Mitochondria comprise up to four ABC systems, ABCB7/ATM1, ABCB10/MDL1, ABCB8 and ABCB6. These half-transporters, which assemble into homodimeric complexes, are involved in a number of key cellular processes, e.g. biogenesis of cytosolic iron–sulfur clusters, heme biosynthesis, iron homeostasis, multidrug resistance, and protection against oxidative stress. We are aiming at understanding how these ABC systems in the inner and outer mitochondrial membrane fulfill their functions in important (patho) physiological processes, including neurodegenerative and hematological disorders.

Relevant Publications:

Zutz, A., Gompf, S., Schägger, H. & Tampé, R. (2009) Mitochondrial ABC-proteins in health and disease.
Biochim. Biophys. Acta - Bioenergetics 1787, 681-690.     PDF

Hofacker, M., Gompf, S., Zutz, A., Presenti, C., Haase, W., van der Does, C., Model, K. & Tampé, R. (2007) Structural and functional fingerprint of the mitochondrial ABC transporter MDL1 from Saccharomyces cerevisiae.
J. Biol. Chem., 282, 33951-61.     PDF

Gompf, S., Zutz, A., Hofacker, M., Haase, W., van der Does, C. & Tampé, R. (2007) Insights in the ABC transporter MDL1 by switching it from posttranslational mitochondrial import to ER insertion. FEBS J. 274, 5298-5310.     PDF

van der Does, C., Presenti, C., Schulze, K., Dinkelaker, S., & Tampé, R. (2006) Kinetics of the ATP hydrolysis cycle of the nucleotide-binding domain of MDL1 studied by a novel site-specific labeling technique.
J. Biol. Chem. 281, 5694-5701.     PDF

van der Does, C. & Tampé, R. (2004) Changing orders – Primary and secondary membrane transporters revised. ChemBioChem 2004, 5, 1171-1175.     PDF

van der Does, C. & Tampé, R. (2004) How do ABC transporters drive transport? Biol. Chem. 385, 927–933.     PDF

Janas, E., Hofacker, M., Chen, M., Gompf, S., van der Does, C. & Tampé, R. (2003) The ATPase Cycle of the nucleotide-binding domain of the mitochondrial ABC transporter Mdl1p. J. Biol. Chem. 278, 26862-26869.     PDF