Group Members

Dr. Robert Tampé
Professor & Director
phone +49-(0)69-798-29476

Susanne Bernhart      
Office Manager
phone +49-(0)69-798-29475

Andrea Pott      
Office Manager
phone +49-(0)69-798-29475

PD Dr. Rupert Abele       
Group Leader  
phone +49-(0)69-798-29437

Dr. Florencia Sánchez
Postdoctoral Fellow
phone +49-(0)69-798-29466

Dr. Christoph Thomas
Postdoctoral Fellow
phone +49-(0)69-798-29486

Dr. Simon Trowitzsch
Group Leader
phone +49-(0)69-798-29273

Dr. Ralph Wieneke
Group Leader
phone +49-(0)69-798-29466

Publications 2017

Blees A, Januliene D, Hofmann T, Koller N, Schmidt C, Trowitzsch S, Moeller A, Tampé R.
Structure of the human MHC-I peptide-loading complex.
Nature. 2017 Nov 6. doi: 10.1038/nature24627.
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Thomas C, Tampé R.
Structure of the TAPBPR-MHC I complex defines the mechanism of peptide loading and editing.
Science. 2017 Oct 12. pii: eaao6001. doi: 10.1126/science.aao6001.
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Khageh Hosseini S, Kolterer S, Steiner M, von Manstein V, Gerlach K, Trojan J, Waidmann O, Zeuzem S, Schulze JO, Hahn S, Steinhilber D, Gatterdam V, Tampé R, Biondi RM, Proschak E, Zörnig M.
Camptothecin and its analog SN-38, the active metabolite of irinotecan, inhibit binding of the transcriptional regulator and oncoprotein FUBP1 to its DNA target sequence FUSE.
Biochem Pharmacol. 2017 Oct 13. pii: S0006-2952(17)30633-0. doi: 10.1016/j.bcp.2017.10.003.
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Heuer A, Gerovac M, Schmidt C, Trowitzsch S, Preis A, Kötter P, Berninghausen O, Becker T, Beckmann R, Tampé R.
Structure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation.
Nat Struct Mol Biol. 2017 May;24(5):453-460. doi: 10.1038/nsmb.3396. Epub 2017 Apr 3.
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Kankate L, Aguf A, Grossmann H, Schnietz M, Tampé R, Turchanin A, Gölzhäuser A.
Vapor phase exchange of self-assembled monolayers for engineering of biofunctional surfaces.
Langmuir. 2017 Mar 24. doi: 10.1021/acs.langmuir.6b04207. [Epub ahead of print]
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Thomas C, Tampé R.
Proofreading of Peptide—MHC Complexes through Dynamic Multivalent Interactions
Front Immunol. 2017 Feb 8;8:65. doi: 10.3389/fimmu.2017.00065. Review.
PMID: 28228754

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Lehnert E, Tampé R.
Structure and Dynamics of Antigenic Peptides in Complex with TAP
Front. Immunol., 30 January 2017 https://doi.org/10.3389/fimmu.2017.00010
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Nöll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour AR, Tomasiak TM, Brüchert S, Joseph B, Abele R, Oliéric V, Wang M, Diederichs K, Hummer G, Stroud RM, Pos KM, Tampé R.
Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP.
Proc Natl Acad Sci U S A. 2017 Jan 9. pii: 201620009. doi: 10.1073/pnas.1620009114. [Epub ahead of print]
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Posch S, Aponte-Santamaría C, Schwarzl R, Karner A, Radtke M, Gräter F, Obser T, König G, Brehm MA, Gruber HJ, Netz RR, Baldauf C, Schneppenheim R, Tampé R, Hinterdorfer P.
Mutual A domain interactions in the force sensing protein von Willebrand factor.

J Struct Biol 2017 197, 57-64. http://dx.doi.org/10.1016/j.jsb.2016.04.012
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Braner M, Wieneke R and Tampé R.
Nanomolar Affinity Protein Trans-Splicing Monitored in Real-Time
by Fluorophore-Quencher Pairs

Chem. Commun., 2017, 545-8. DOI: 10.1039/C6CC08862G.
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Publications 2016

Herbring V, Bäucker A,Trowitzsch S, Tampé R
A dual inhibition mechanism of herpesviral ICP47 arresting a conformationally thermostable TAP complex
Scientific Reports 6, 36907
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Kiosze-Becker K, Ori A, Gerovac M, Heuer A, Nürenberg-Goloub E, Jan Rashid U,
Becker T, Beckmann R, Beck M, Tampé R,
Structure of the ribosome post-recycling complex probed by chemical cross-linking and mass spectrometry
Nature Communications 2016/11/08/online
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Robb NC, Te Velthuis AJ, Wieneke R, Tampé R, Cordes T, Fodor E, Kapanidis AN.
Single-molecule FRET reveals the pre-initiation and initiation conformations of influenza virus promoter RNA.
Nucleic Acids Res. 2016 Sep 30. pii: gkw884.
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Lehnert E, Mao J, Mehdipour AR, Hummer G, Abele R, Glaubitz C, Tampé R.
Antigenic peptide recognition on the human ABC transporter TAP resolved by DNP-enhanced solid-state NMR spectroscopy.
J Am Chem Soc. 2016 Sep 23. [Epub ahead of print]
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Guesdon A, Bazile F, Buey RM, Mohan R, Monier S, García RR, Angevin M, Heichette C, Wieneke R, Tampé R, Duchesne L, Akhmanova A, Steinmetz MO, Chrétien D.
EB1 interacts with outwardly curved and straight regions of the microtubule lattice.
Nat Cell Biol. 2016 Oct;18(10):1102-8. doi: 10.1038/ncb3412.[Epub ahead of print]
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Casanas, A, Warshamanage, R, Finke, A D, Panepucci, E, Olieric, V, Noll, A, Tampe, R, Brandstetter, S, Forster, A, Mueller, M, Schulze-Briese, C, Bunk, O & Wang, M.
EIGER detector: application in macromolecular crystallography
Acta Crystallographica Section D 2016;72(9):1036-1048doi:10.1107/S2059798316012304
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Posch S, Aponte-Santamaría C, Schwarzl R, Karner A, Radtke M, Gräter F, Obser T, König G, Brehm MA, Gruber HJ, Netz RR, Baldauf C, Schneppenheim R, Tampé R, Hinterdorfer P.
Single molecule force spectroscopy data and BD- and MD simulations on the blood protein von Willebrand factor.
Data Brief. 2016 Jul 21;8:1080-7. doi: 10.1016/j.dib.2016.07.031. eCollection 2016.
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Michael Urban, Marc Vor der Brüggen, Robert Tampé.
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip Systemat Single Protein Resolution
J Vis Exp. 2016 Aug 16;(114). doi: 10.3791/53373.
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Schmidt A, Brettschneider K, Kahle J, Orlowski A, Becker-Peters K, Stichel D, Schulze J, Braner M, Tampé R, Schwabe D, Königs C.
Neutralisation of factor VIII inhibitors by anti-idiotypes isolated from phage-displayed libraries.
Thromb Haemost. 2016 Mar 24;116(1). [Epub ahead of print]
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Braner M, Kollmannsperger A, Wieneke R and Tampé R. 
‘Traceless’ tracing of proteins – high-affinity trans-splicing directed by a minimal interaction pair
Chem. Sci., 2016, 7, 2646-2652 DOI: 10.1039/C5SC02936H
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Kollmannsperger A, Sharei A, Raulf A, Heilemann M, Langer R, Jensen KF, Wieneke R, Tampé R.
Live-cell protein labelling with nanometre precision by cell squeezing.
Nat Commun. 2016 Jan 29;7:10372. doi: 10.1038/ncomms10372.
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Fisette O, Wingbermühle S, Tampé R, Schäfer LV.
Molecular mechanism of peptide editing in the tapasin-MHC I complex.
Sci Rep. 2016 Jan 12;6:19085. doi: 10.1038/srep19085.
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Heise R, Amann PM, Ensslen S, Marquardt Y, Czaja K, Joussen S, Beer D, Abele R, Plewnia G, Tampé R, Merk HF, Hermanns HM, Baron JM.
Interferon Alpha Signalling and Its Relevance for the Upregulatory Effect of Transporter Proteins Associated with Antigen Processing (TAP) in Patients with Malignant Melanoma.
PLoS One. 2016 Jan 6;11(1):e0146325. doi: 10.1371/journal.pone.0146325. eCollection 2016.