Dr. Eric R. Geertsma
Junior Professor
Biocenter N200/1.08
Phone +49-(0)69-798-29255
ax: +49-(0)69-798 29244


Beate Braungart
Administrative assistant
Biocenter N200/1.10
Phone +49-(0)69-798-29238
Fax: +49-(0)69-798 29244


All publications

Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER*, Dawson RJ*, Seeger MA*.
Synthetic single domain antibodies for the conformational trapping of membrane proteins.
Elife. 2018 May 24;7. pii: e34317

Koch K, Kalusche S, Torres JL, Stanfield RL, Danquah W, Khazanehdari K, von Briesen H, Geertsma ER, Wilson IA, Wernery U, Koch-Nolte F, Ward AB, Dietrich U. 
Selection of nanobodies with broad neutralizing potential against primary HIV-1 strains using soluble subtype C gp140 envelope trimers. 
Sci Rep. 2017 Aug 21;7(1):8390.

Marino J, Holzhüter K, Kuhn B, Geertsma ER. 
Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli
Methods Enzymol. 2017;594:139-164.

Schenck S, Kunz L, Sahlender D, Pardon E, Geertsma ER, Savtchouk I, Suzuki T, Neldner Y, Štefanić S, Steyaert J, Volterra A, Dutzler R. 
Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport. 
Biochemistry. 2017 Aug 1;56(30):3962-3971.

Chang YN, Geertsma ER. 
The novel class of seven transmembrane segment inverted repeat carriers. 
Biol Chem. 2017 Feb 1;398(2):165-174.

Geertsma ER, Chang YN, Shaik FR, Neldner Y, Pardon E, Steyaert J, Dutzler R.
Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family.
Nat Struct Mol Biol. 2015 Oct;22(10):803-8.

Bukowska MA, Hohl M, Geertsma ER, Hürlimann LM, Grütter MG, Seeger MA.
A Transporter Motor Taken Apart: Flexibility in the Nucleotide Binding Domains of a Heterodimeric ABC Exporter.
Biochemistry. 2015 May 19;54(19):3086-99.

Marino J, Hohl M, Seeger MA, Zerbe O, Geertsma ER.
Bicistronic mRNAs to enhance membrane protein overexpression.
J Mol Biol. 2015 Feb 27;427(4):943-54

Ehrnstorfer IA, Geertsma ER, Pardon E, Steyaert J, Dutzler R.
Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.
Nat Struct Mol Biol. 2014 Nov;21(11):990-6

Geertsma ER.
FX cloning: a simple and robust high-throughput cloning method for protein expression.
Methods Mol Biol. 2014;1116:153-64.

Geertsma ER.
FX cloning: a versatile high-throughput cloning system for characterization of enzyme variants.
Methods Mol Biol. 2013;978:133-48.

Marino J, Geertsma ER, Zerbe O.
Topogenesis of heterologously expressed fragments of the human Y4 GPCR.

Biochim Biophys Acta. 2012 Dec;1818(12):3055-63.

Malojčić G*, Geertsma ER*, Brozzo MS, Glockshuber R.
Mechanism of the prokaryotic transmembrane disulfide reduction pathway and its in vitro reconstitution from purified components.
Angew Chem Int Ed Engl. 2012 Jul 9;51(28):6900-3.

Marreddy RK, Pinto JP, Wolters JC, Geertsma ER, Fusetti F, Permentier HP, Kuipers OP, Kok J, Poolman B.
The response of Lactococcus lactis to membrane protein production.

PLoS One. 2011;6(8):e24060.

Geertsma ER, Dutzler R.
A versatile and efficient high-throughput cloning tool for structural biology.
Biochemistry. 2011 Apr 19;50(15):3272-8.

Linares DM, Geertsma ER, Poolman B.
Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins.
J Mol Biol. 2010 Aug 6;401(1):45-55.

Marreddy RK, Geertsma ER, Permentier HP, Pinto JP, Kok J, Poolman B.
Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.
PLoS One. 2010 Apr 26;5(4):e10317.

Geertsma ER, Poolman B.
Production of membrane proteins in Escherichia coli and Lactococcus lactis.

Methods Mol Biol. 2010;601:17-38.

Kuiper JM, Pluta R, Huibers WH, Fusetti F, Geertsma ER, Poolman B.
A method for site-specific labeling of multiple protein thiols.

Protein Sci. 2009 May;18(5):1033-41.

Mulligan C*, Geertsma ER*, Severi E, Kelly DJ, Poolman B, Thomas GH.
The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter.

Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1778-83.

Geertsma ER, Groeneveld M, Slotboom DJ, Poolman B.
Quality control of overexpressed membrane proteins.
Proc Natl Acad Sci U S A. 2008 Apr 15;105(15):5722-7.

Geertsma ER, Nik Mahmood NA, Schuurman-Wolters GK, Poolman B.
Membrane reconstitution of ABC transporters and assays of translocator function.
Nat Protoc. 2008;3(2):256-66.

Geertsma ER, Poolman B.
High-throughput cloning and expression in recalcitrant bacteria.

Nat Methods. 2007 Sep;4(9):705-7.

Poolman B, Geertsma ER, Slotboom DJ. Biochemistry.
A missing link in membrane protein evolution.

Science. 2007 Mar 2;315(5816):1229-31.

Duurkens RH, Tol MB, Geertsma ER, Permentier HP, Slotboom DJ.
Flavin binding to the high affinity riboflavin transporter RibU.

J Biol Chem. 2007 Apr 6;282(14):10380-6.

Burgess CM, Slotboom DJ, Geertsma ER, Duurkens RH, Poolman B, van Sinderen D.
The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism.

J Bacteriol. 2006 Apr;188(8):2752-60.

Poolman B, Doeven MK, Geertsma ER, Biemans-Oldehinkel E, Konings WN, Rees DC.
Functional analysis of detergent-solubilized and membrane-reconstituted ATP-binding cassette transporters.

Methods Enzymol. 2005;400:429-59.

Geertsma ER, Duurkens RH, Poolman B.
The activity of the lactose transporter from Streptococcus thermophilus is increased by phosphorylated IIA and the action of beta-galactosidase.
Biochemistry. 2005 Dec 6;44(48):15889-97.

Geertsma ER, Duurkens RH, Poolman B.
Functional interactions between the subunits of the lactose transporter from Streptococcus thermophilus.
J Mol Biol. 2005 Jul 1;350(1):102-11.

Vervoort EB, Bultema JB, Schuurman-Wolters GK, Geertsma ER, Broos J, Poolman B.
The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane.

J Mol Biol. 2005 Feb 25;346(3):733-43.

Doeven MK, Folgering JH, Krasnikov V, Geertsma ER, van den Bogaart G, Poolman B.
Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles.
Biophys J. 2005 Feb;88(2):1134-42.

Geertsma ER, Duurkens RH, Poolman B.
Identification of the dimer interface of the lactose transport protein from Streptococcus thermophilus.
J Mol Biol. 2003 Oct 3;332(5):1165-74.

Veenhoff LM, Geertsma ER, Knol J, Poolman B.
Close approximation of putative alpha -helices II, IV, VII, X, and XI in the translocation pathway of the lactose transport protein of Streptococcus thermophilus.
J Biol Chem. 2000 Aug 4;275(31):23834-40.