Publications 2015

Blees A, Reichel K, Trowitzsch S, Fisette O, Bock C, Abele R, Hummer G, Schäfer LV, Tampé R.
Assembly of the MHC I peptide-loading complex determined by a conserved ionic lock-switch.
Sci Rep. 2015 Nov 27;5:17341. doi: 10.1038/srep17341.

Urban M, Tampé R.
Membranes on nanopores for multiplexed single-transporter

Microchim Acta, DOI 10.1007/s00604-015-1676-4

Pfreundschuh M, Alsteens D, Wieneke R, Zhang C, Coughlin SR, Tampé R, Kobilka BK, Müller DJ.
Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM.
Nat Commun. 2015 Nov 12;6:8857. doi: 10.1038/ncomms9857.

Fleischmann G, Fisette O, Thomas C, Wieneke R, Tumulka F, Schneeweiss C, Springer S, Schäfer LV, Tampé R.
Mechanistic Basis for Epitope Proofreading in the Peptide-Loading Complex.
J Immunol. 2015 Sep 28. pii: 1501515.

Wieneke R, Raulf A, Kollmannsperger A, Heilemann M, Tampé R.
SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging.
Angew Chem Int Ed Engl. 2015 Aug 24;54(35):10216-9. doi: 10.1002/anie.201503215. Epub 2015 Jul 15.

Strale PO, Duchesne L, Peyret G, Montel L, Nguyen T, Png E, Tampé R, Troyanovsky S, Hénon S, Ladoux B, Mège RM.
The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity.
J Cell Biol. 2015 Jul 20;210(2):333-46. doi: 10.1083/jcb.201410111.

Koho T, Ihalainen TO, Stark M, Uusi-Kerttula H, Wieneke R, Rahikainen R, Blazevic V, Marjomäki V, Tampé R, Kulomaa MS, Hytönen VP.
His-tagged norovirus-like particles: A versatile platform for cellular delivery and surface display.
Eur J Pharm Biopharm. 2015 Jul 10. pii: S0939-6411(15)00290-8. doi: 10.1016/j.ejpb.2015.07.002. [Epub ahead of print]

Trowitzsch S, Tampé R.
Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes.
Methods Enzymol. 2015;557:3-26. doi: 10.1016/bs.mie.2014.11.043. Epub 2015 Jan 10.

Tampé R.
Membrane Transport and Communication in Frankfurt: Speakers' Summary - Highlights.
Biol Chem. 2015 Sep 1;396(9-10):949-54. doi: 10.1515/hsz-2015-0211.

Kemmer GC, Bogh SA, Urban M, Palmgren MG, Vosch T, Schiller J, Günther Pomorski T.
Lipid-conjugated fluorescent pH sensors for monitoring pH changes in reconstituted membrane systems.
Analyst. 2015 Aug 24;140(18):6313-20. doi: 10.1039/c5an01180a.

Eggensperger S, Tampé R.
The transporter associated with antigen processing: a key player in adaptive immunity.
Biol Chem. 2015 Sep 1;396(9-10):1059-72. doi: 10.1515/hsz-2014-0320.

Zollmann T, Bock C, Graab P, Abele R.
Team work at its best - TAPL and its two domains.
Biol Chem. 2015 Feb 25. pii: /j/bchm.just-accepted/hsz-2014-0319/hsz-2014-0319.xml. doi: 10.1515/hsz-2014-0319. [Epub ahead of print]

Mayerhofer PU, Tampé R.
Antigen Translocation Machineries in Adaptive Immunity and Viral Immune Evasion.
J Mol Biol. 2015 Mar 13;427(5):1102-1118. doi: 10.1016/j.jmb.2014.09.006. Epub 2014 Sep 16.

Thiele F, Tao S, Zhang Y, Muschaweckh A, Zollmann T, Protzer U, Abele R, Drexler I.
Modified Vaccinia Virus Ankara-Infected Dendritic Cells Present CD4+ T-Cell Epitopes by Endogenous Major Histocompatibility Complex Class II Presentation Pathways.
J Virol. 2015 Mar 1;89(5):2698-709. doi: 10.1128/JVI.03244-14. Epub 2014 Dec 17.

Fischbach H, Döring M, Nikles D, Lehnert E, Baldauf C, Kalinke U, Tampé R.
Ultrasensitive quantification of TAP-dependent antigen compartmentalization in scarce primary immune cell subsets.
Nat Commun. 2015 Feb 6;6:6199. doi: 10.1038/ncomms7199.

Bechara C, Nöll A, Morgner N, Degiacomi MT, Tampé R*, Robinson CV*.
A subset of annular lipids is linked to the flippase
activity of an ABC transporter.

Nat Chem. 2015 Mar;7(3):255-62. doi: 10.1038/nchem.2172. Epub 2015 Feb 2.

News & Views

             Harvey SR, Wysocki VH.
             Mass spectrometry: Bound in flight
             Nat Chem. 2015 2 (7) 189–190 DOI: 10.1038/NCHEM.2192

Zollmann T, Moiset G, Tumulka F, Tampé R, Poolman B, Abele R.
Single liposome analysis of peptide translocation by the ABC transporter TAPL.
Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):2046-51. doi: 10.1073/pnas.1418100112. Epub 2015 Feb 2.

Kim J, Wu S, Tomasiak TM, Mergel C, Winter MB, Stiller SB, Robles-Colmanares Y, Stroud RM*, Tampé R*, Craik CS*, Cheng Y*.
Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter.
Nature. 2015 Jan 15;517(7534):396-400. doi: 10.1038/nature13872. Epub 2014 Nov 2.

Publications 2014

Lin J, Eggensperger S, Hank S, Wycisk A, Wieneke R, Mayerhofer PU, Tampé R
A negative feedbackmodulator of antigen processing evolved from a frameshift in the cowpox virus genome.
PLoS Pathog. 2014 Dec 11,DOI: 10.1371/journal.ppat.1004554

Grossmann N, Vakkasoglu AS, Hulpke S, Abele R, Gaudet R, Tampé R.
Mechanistic determinants of the directionality and energetics of active export by a heterodimeric ABC transporter.
Nat Commun. 2014 Nov 7;5:5419. doi: 10.1038/ncomms6419.

Kim J, Wu S, Tomasiak TM, Mergel C, Winter MB, Stiller SB, Robles-Colmanares Y, Stroud RM, Tampé R, Craik CS, Cheng Y.
Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter.
Nature. 2014 Nov 2. doi: 10.1038/nature13872. [Epub ahead of print]

Hinz A, Jedamzick J, Herbring V, Fischbach H, Hartmann J, Parcej D, Koch J, Tampé R.
Assembly and function of the MHC I peptide-loading complex are conserved across higher vertebrates
J. Biol. Chem. 2014 Oct 15; 289: 33109-33117.

Eggensperger S, Fisette O, Parcej D, Schäfer LV, Tampé R.
An annular lipid belt is essential for allosteric coupling and viral inhibition of the antigen translocation complex TAP.
J. Biol. Chem. 2014; 289: 33098-33108.

Wieneke R, Laboria N , Rajan M, Kollmannsperger A, Natale F, Cardoso MC , Tampé R.
Live-cell targeting of His-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes
J. Am. Chem. Soc., 2014, 136 (40), pp 13975–13978 DOI: 10.1021/ja5063357.

Mayerhofer PU, Tampé R.
Antigen Translocation Machineries in Adaptive Immunity and Viral Immune Evasion.
Mol Biol. 2014 Sep 12. pii: S0022-2836(14)00493-8. doi: 10.1016/j.jmb.2014.09.006.

Nair-Gupta P, Baccarini A, Tung N, Seyffer F, Florey O, Huang Y, Banerjee M, Overholtzer M, Roche PA, Tampé R, Brown BD, Amsen D, Whiteheart SW, Blander JM.
TLR Signals Induce Phagosomal MHC-I Delivery from the Endosomal Recycling Compartment to Allow Cross-Presentation
Cell. 2014 July 31;158(6):506–21. doi: 10.1016/j.cell.2014.04.054

Seyffer F, Tampé R.
ABC transporters in adaptive immunity.
Biochim Biophys Acta. 2014 Jun 9. pii: S0304-4165(14)00220-7. doi: 10.1016/j.bbagen.2014.05.022.

Arnold P, Himmels P, Weiß S, Decker TM, Markl J, Gatterdam V, Tampé R, Bartholomäus P, Dietrich U, Dürr R1.
Antigenic and 3D structural characterization of soluble X4 and hybrid X4-R5 HIV-1 Env trimers.
Retrovirology. 2014 May 30;11(1):42. doi: 10.1186/1742-4690-11-42.

Gatterdam V, Ramadass R, Stoess T, Fichte MA, Wachtveitl J, Heckel A, Tampé R.
Three-Dimensional Protein Networks Assembled by Two-Photon Activation.
Angew Chem Int Ed Engl. 2014 May ;53(22):5680-84.

Gatterdam V1, Ramadass R, Stoess T, Fichte MA, Wachtveitl J, Heckel A, Tampé R.
Dreidimensionale Proteinnetzwerke durch Zwei-Photonen-Aktivierung
Angew Chem 2014 May 126(22):5787-91.

Urban M, Kleefen A, Mukherjee N, Seelheim P, Windschiegl B, von der Brüggen M, Kocer A, Tampé R.
Highly parallel transport recordings on a membraneon-nanopore chip at single molecule resolution.
Nano Lett. 2014 Mar 12;14(3):1674-80. doi: 10.1021/nl5002873. Epub 2014 Feb 17.