Research Centers

Collaborative Research Center SFB 807
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Cluster of Excellence Frankfurt CEF - Macromolecular Complexes
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Collaborative Research Center SFB 902
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Complex Scenarios of Light Control
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Plasmids and Strains

Plasmids and strains for use in C. elegans Optogenetics:

all exisiting in various expression plasmids for C. elegans, with different fluorescent proteins, and using different promoters (details available upon request)

  • ChR2 WT: wild-type Channelrhodopsin
  • ChR2(H134R): "stabilized"; "g.o.f." ChR2
  • ChR2(H134R, T159C): improved conductance and expression
  • ChR2(C128S): "slow" ChR2 (aka as "step-function opsin")
  • C1V1-ET/ET: red-shifted action spectrum
  • ChR2(L132C): Ca2+ transporting ChR (aka as "CatCh")

Halorhodopsin and other Hyperpolarizers:

  • NpHR: Halorhodopsin from Natronomonas pharaonis
  • Mac: Proton pump from Leptosphaeria maculans
  • Arch: archaerhodopsin-3, proton pump from Halorubrum sodomense

Photoactivated adenyly cyclase (PAC)

  • PAC from Euglena gracilis

Vectors for single-cell expression using intersecting promoters

  • cre recombinase
  • loxP-STOP-LacZ-loxP::ChR2-SL2-GFP operon
  • FLP recombinase
  • FRT-mCherry-STOP-FRT::ChR2::YFP

Integrated transgenes

  • zxIs3[punc-47::ChR2(H134R)::YFP; lin-15+]I: GABAergic neurons
  • zxIs5[punc-17::ChR2(H134R)::YFP; lin-15+]X: cholinergic neurons
  • zxIs6[punc-17::ChR2(H134R)::YFP; lin-15+]V: cholinergic neurons
  • zxIs12[pF49H12.4::ChR2::mCherry; pF49H12.4::GFP]: PVD, AQR, tail neuron

A Matlab script based on the "Parallel Worm Tracker" by Ramot et al. (2008, PLoS ONE 3: e2208), that incorporates the use of a light source and a shutter, to allow photostimulation of transgenic neurons in freely behaving animals. Modified /written by Wagner Steuer Costa ().

The software is free to use, but please acknowledge this accordingly upon publication.

Download (.rar Archive, includes file "120206 WSC OptoTracker Info" with instructions for installation and use).